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Stem Cell Therapy For Dogs | MSAH – Metairie Small Animal …

Stem cell technology is a new and exciting branch of veterinary medicine. Stem cells are cells that can be safely harvested from a variety of adult animal tissues (primarily fat tissue). Once harvested, the stem cells can be injected and induced to grow into a large number of different cell types.

Stem cell therapy offers the possibility of using these stem cells to grow into replacements for injured or diseased tissues such as bone, cartilage, muscle, nerves, and so on. What makes this such an exciting treatment option is that it allows us to introduce real tissue replacements for damaged tissues, rather than artificial replacement implants. Currently stem cell therapy for dogs is primarily being used with some success to treat bone, joint,ligament problems and autoimmune conditions.

The technology involves the use of adult stem cells, which can be easily obtained, without any harm to the host animal from which they are taken. A small amount of adipose (fat) tissue is surgically removed from a host animal and a centrifuge is used to separate the stem cells from the fat tissue. The tissue does have to be removed surgically, so if you are considering stem cell therapy later in your pet’s life, we recommend obtaining the tissue during another procedure, such as a spay or neuter. The stem cells collected are then injected directly into the injured joint, ligament, or bone where they quickly begin to grow, potentially replacing the damaged tissue with new, healthy tissue.

Stem cell therapy holds immense promise for becoming a viable treatment option for a variety of health issues dogs face.

At Metairie Small Animal Hospital, we believe that providing your canine companion with the highest quality medical care means being on the cutting edge of the latest technologies and procedures. This is reflected in every aspect of our approach to stem cell therapy for dogs. Our facilities feature state-of-the-art equipment, and our veterinary team stays up to date on the latest canine stem cell research available.

The ideal candidate for canine stem cell therapy is a dog in otherwise good health that suffers from arthritis or hip dysplasia, and who doesn’t respond well to his or her medication. Or, a dog whose quality of life might further suffer due to invasive surgical procedures. Because canine stem cell therapy uses the patient’s own tissues, a canine must be in overall good health in order for any collected stem cells to be effective.

Canine stem cells are collected through removing either fat cells, or various other applicable tissues from a dog’s body. Within these tissues and cells exist regenerative cells that are known colloquially as dog stem cells. The regenerative cells that are collected do several things:

The best part is that canine stem cells are not synthetic cells being added to a living, biological organism. Rather, canine stem cells are a dog’s own natural healing cells.Because of this, there is much less chance of rejection or adverse interaction, and there are also fewer potential side effects.

Due to the infancy of canine stem cell research and therapies, there is not yet a large body of information about possible adverse side effects. Just like any medical procedure, the risk for adverse side effects from dog stem cell therapy are ever-present. Risks could be associated with the tissue removal procedure, or from a patient’s body rejecting the newly placed cells. Other issues with stem cell therapy for dogs include its effectiveness when used alone. This is because the prescription of traditional medication will accompany stem cell therapy procedures.

Deciding whether or not stem cell therapy for dogs is the right choice for your beloved canine companion is a very personal one. No matter what route you choose to take, we are here to help you make the decision that is in the best interest of your dog, and alsofits your budget and lifestyle.

At this time, most stem cell research for dogs currently focuses on treating bone, joint, and ligament problems, rather than treating more advanced illnesses and diseases. We are extremely confident that the day will come when many canine illnesses and diseases can be treated through advances in canine stem cell research.

One thing seems to be clear, stem cell research for dogs promises to revolutionize the veterinary industry, and is already showing good results with canines that have been treated with dog stem cells to repair joints, bones, or ligaments that have been damaged by injury or disease. We are here to help educate you about the latest canine stem cell research advances, and answer any other questions about dog stem cell therapy you might have.

If you would like to discuss how stem cell therapy can benefit your dog, please contact us to schedule an appointment. Our veterinary team can help you decide whether or not canine stem cell therapy is the right option, including discussing cost and prognosis.

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Stem Cell Therapy For Dogs | MSAH – Metairie Small Animal …

Wound licking – Wikipedia

Wound licking is an instinctive response in humans and many other animals to lick an injury. Dogs, cats, small rodents, horses, and primates all lick wounds.[1] Saliva contains tissue factor which promotes the blood clotting mechanism. The enzyme lysozyme is found in many tissues and is known to attack the cell walls of many gram-positive bacteria, aiding in defense against infection. Tears are also beneficial to wounds due to the lysozyme enzyme. However, there are also infection risks due to bacteria in the human mouth.

Oral mucosa heals faster than skin,[2] suggesting that saliva may have properties that aid wound healing. Saliva contains cell-derived tissue factor, and many compounds that are antibacterial or promote healing. Salivary tissue factor, associated with microvesicles shed from cells in the mouth, promotes wound healing through the extrinsic blood coagulation cascade.[3][4][5] The enzymes lysozyme and peroxidase,[6] defensins,[7] cystatins and an antibody, IgA,[8] are all antibacterial. Thrombospondin and some other components are antiviral.[9][10] A protease inhibitor, secretory leukocyte protease inhibitor, is present in saliva and is both antibacterial and antiviral, and a promoter of wound healing.[11][12] Nitrates that are naturally found in saliva break down into nitric oxide on contact with skin, which will inhibit bacterial growth.[13] Saliva contains growth factors[14] such as epidermal growth factor,[15] VEGF,[16] TGF-1,[17] leptin,[18][19] IGF-I,[20][21] lysophosphatidic acid,[22][23] hyaluronan[24] and NGF,[25][26][27] which all promote healing, although levels of EGF and NGF in humans are much lower than those in rats. In humans, histatins may play a larger role.[28][29] As well as being growth factors, IGF-I and TGF- induce antimicrobial peptides.[30] Saliva also contains an analgesic, opiorphin.[31] Licking will also tend to debride the wound and remove gross contamination from the affected area.In a recent study, scientists have confirmed through several experiments that the protein responsible for healing properties in human saliva is, in fact, histatin. Scientists are now looking for ways to make use of this information in ways that can lead to chronic wounds, burns, and injuries being healed by saliva, making this type of treatment as common as antibiotic creams and rubbing alcohol.[32]

It has been long observed that the licking of their wounds by dogs might be beneficial. Indeed, a dog’s saliva is bactericidal against the bacteria Escherichia coli and Streptococcus canis, although not against coagulase positive Staphylococcus or Pseudomonas aeruginosa.[33]Wound licking is also important in other animals. Removal of the salivary glands of mice[34] and rats slows wound healing, and communal licking of wounds among rodents accelerates wound healing.[35][36] Communal licking is common in several primate species. In macaques, hair surrounding a wound and any dirt is removed, and the wound is licked, healing without infection.[37]

Wound licking is beneficial but too much licking can be harmful. An Elizabethan collar may be used on pet animals to prevent them from biting an injury or excessively licking it, which can cause a lick granuloma. These lesions are often infected by pathogenic bacteria such as Staphylococcus intermedius.[38] Infection is another risk. Horses that lick wounds may become infected by a stomach parasite, Habronema, a type of nematode worm. The rabies virus may be transmitted between animals, such as the kudu antelopes by wound licking of wounds with residual infectious saliva.[39]

There are many legends involving healing wounds by licking them or applying saliva. Saint Magdalena de Pazzi is said to have cured a nun of sores and scabs in 1589 by licking her limbs.[40] The Roman Emperor Vespasian is said to have performed a healing of a blind man using his saliva.[41] Pliny the Elder in his Natural History reported that a fasting woman’s saliva is an effective cure for bloodshot eyes.

There are potential health hazards in wound licking due to infection risk, especially in immunocompromised patients. Human saliva contains a wide variety of bacteria that are harmless in the mouth, but that may cause significant infection if introduced into a wound. A notable case was a diabetic man who licked his bleeding thumb following a minor bicycle accident, and subsequently had to have the thumb amputated after it became infected with Eikenella corrodens from his saliva.[42] The practice of metzitzah during circumcision is controversial as it can transmit the herpes virus to the infant.[43]

Dog saliva has been said by many cultures to have curative powers in people.[44][45] “Langue de chien, langue de mdecin” is a French saying meaning “A dog’s tongue is a doctor’s tongue”, and a Latin quote that “Lingua canis dum lingit vulnus curat” or “A dog’s saliva can heal your wound” appears in a thirteenth-century manuscript.[46] In Ancient Greece, dogs at the shrine of Aesculapius were trained to lick patients, and snake saliva was also applied to wounds.[47] Saint Roch in the Middle Ages was said to have been cured of a plague of sores by licking from his dog.[48] The Assyrian Queen Semiramis is supposed to have attempted to resurrect the slain Armenian king Ara the Beautiful by having the dog god Aralez lick his wounds.[49] In the Scottish Highlands in the nineteenth century, dog lick was believed to be effective for treating wounds and sores.[50] In the Gospel of Luke (16:19-31), Lazarus the Beggar’s sores are licked by dogs, although no curative effects are reported by the Evangelist.

There are contemporary reports of the healing properties of dog saliva. Fijian fishermen are reported to allow dogs to lick their wounds to promote healing,[13] and a case of dog saliva promoting wound healing was reported in the Lancet medical journal.[51]

As with the licking of wounds by people, wound licking by animals carries a risk of infection. Allowing pet cats to lick open wounds can cause cellulitis[52][53] and septicemia[54][55] due to bacterial infections. Licking of open wounds by dogs could transmit rabies if the dog is infected with rabies,[56] although this is said by the CDC to be rare.[57] Dog saliva has been reported to complicate the healing of ulcers.[58] Another issue is the possibility of an allergy to proteins in the saliva of pets, such as Fel d 1 in cat allergy and Can f 1 in dog allergy.[59] Cases of serious infection following the licking of wounds by pets include:

To “lick your wounds” means to “withdraw temporarily while recovering from a defeat”[70]

The phrase was spoken by Antony in John Dryden’s seventeenth century play All for Love:[71]

They look on us at distance, and, like cursScaped from the lion’s paws, they bay far offAnd lick their wounds, and faintly threaten war.

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Wound licking – Wikipedia

Stem Cell Therapy for Skin Allergy in Dogs and Cats (Pets …

Stem Cell Therapy for skin allergy in Dogs and Cats (before and after), treated by Dr. Kraemeer

Stem Cell Therapy for skin allergies in dogs and cats is now being offered by Dr. Kraemer (DrKraemers.com)Vet4HealthyPet Advanced Medical Care Orange County California Location (Vet4HealthyPet.com). Stem Cell Therapy (regenerative medicine) for dogs and cats has been extensively researched for itspain relief, anti-arthritis, anti-inflammatory and immune modulation properties.

Atopic allergic itch dermatitis is one of the most common skin allergies in dogs and cats. Allergic itch skin dermatitis in pets is usually manifested by the dog or cat itching, biting and causing self-trauma which then leads to hair loss (alopecia) and often secondary bacterial skin infection (pyoderma) and secondary yeast infection (Malassezia).Stem Cell Therapy for skin allergies in Dogs and Cats is showing great therapeutic promise. (StemCell4Pet.com)

Atopic allergic skin dermatitis conjures up thoughts of stressed animals, unhappy owners and frustrated veterinarians. Atopic itch allergic dermatitis in pets is possibly the number one reason for a veterinary office visit for a dog or cat owner. Atopic itch skin dermatitis in pets can be chronic and often requires treatment over an extended period of time, thus a major financial burden to the pet owner.

Stem cells are powerful healing cells that both you and your pet naturally store in a dormant form. Yourdog or cats stem cells are extracted from their fat (adipose tissue) among a mixture of cells termed the Stromal Vascular Fraction (SVF). The SVF is rich in complementary cells and bioactive peptides that contribute to cell proliferation and tissue regeneration.

Dr. Kraemers in house stem cell therapy isolates stem cells from your dog or cats fatty tissue, then activates them (placing the cells into a hyperactive mode) and within hours, reintroduces them to injured and needed body parts.

Stem Cell Therapy for skin allergy in Dogs and Cats before treatment (Severe pododermatitis)

Stem Cell Therapy for skin allergy in Dogs and Cats after treatment by Dr. Kraemer

In contrast to your pets food allergies, avoiding implicated outdoors allergens (grass, weed, trees etc.) and even indoor allergies (mold, dust mites, human dander etc.) is usually difficult to accomplish. Traditionally, most dogs and cats who suffer from allergic itching atopic skin dermatitis are prescribed medication to help modify their symptoms (i.e. anti itching and biting rx). Additional medication can be prescribed to help decrease the secondary infections (bacteria and yeast). Most of the traditional prescribed drugs lack effectiveness and have the potential for accumulative, adverse effects. In addition, over time they become costly and time consuming, thus reducing the owners compliance.Dr. Kraemer has been offering stem cell therapy for dogs and cats for nearly a decade and is a leading provider of a number of non-pharmaceutical/alternative therapeutic modalities. These new age, non-pharmaceutical alternatives such as Laser therapy, Pulse Electromagnetic Therapy (PEMT), and Platelet Rich Plasma (PRP) can all help manage your pets chronic pain andarthritis, as well as assist in treating a wide range of animal diseases and medical conditions. Dr. Kraemer also offers a low cost Cryobanking programs for future stem cell treatments.

Stem Cell Therapy for atopic allergic itch dermatitis in pets is low risk since you are reintroducing your pets own natural repair cells. Dr. Kraemers in-house stem cell therapy service has the advantage over other stem cell providers because the extracting, processing, and treating is all done on location the same day, thus eliminating unnecessary and unwanted cell death due to transportation and time gap between extraction and treatment.

Stem Cell Therapy for atopic itch Skin Allergy in dogs and cats before & after photos treated by Dr. Kraemer

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #1: Any pet, dog or cat when presented with itching, red irritated skin, patchy hair, bumps, color change and thickness of the effected skin, should suspect allergic atopic itch dermatitis to be the cause.

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #2:Allergic atopic itch dermatitis in most pets, dogs and cats is localized to the ears, paws, legs, arm pits and abdomen.

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #3:Common allergens effecting dogs and cats suspected of itch atopic allergic dermatitis include grass, weeds and trees as well as indoor allergens like mold, dust mites and human dandruff.

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and CatsTip #4:For your petswith itching and allergy dermatitis who are under a year old , I recommend an elimination dietary trial to rule out food allergies

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #5: Platelet Rich Plasma (PRP) allows for an adjunctive autologous value to be added to the stem cell fraction. Platelet Rich Plasma is derived from an autologous whole blood sample from the patient and once processed yields many healing growth factors. PRP can help with survival of the cells upon introducing to the patient, reduce inflammation as well as provide cyto-protective properties when transit is necessary.

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #6:You should use a flea preventative on your pet especially in geographic location where fleas are common.

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #7: OTC antihistamines can be used for dog skin allergies like atopic itch dermatitis but its therapeutic effect is questionable.

2 months post stem cell therapy treatment for a bulldog with a severe form of atopic allergic dermatitis

Dr. Kraemers Stem Cell Therapy for skin allergy in Dogs and Cats Tip #8:Treating allergic atopic itch dermatitis should involvebathing your pet with medicated shampoos and conditioners like Dr. Kraemers V4B Itchy Skin Medicated Shampoo as well as frequent use of medicated lotions like Dr. Kraemer V4B waterless lotions line. I usually also recommend topical medicated shampoos like Dr. Kraemers V4B Antiseptic Medicated Shampoo and V4B Yeasty Lotion to help manage the secondary infection.

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Stem Cell Therapy for Skin Allergy in Dogs and Cats (Pets …

Stem Cell Therapy For Dogs – What you should know

Stem cell technology is a new and exciting branch of veterinary medicine. Stem cells are cells that can be safely harvested from a variety of adult animal tissues. Once harvested, the stem cells can be injected and induced to grow into a large number of different cell types.

Stem cell therapy offers the possibility of using these stem cells to grow into replacements for injured or diseased tissues such as bone, cartilage, muscle, nerves, and so on. What makes this such an exciting treatment option is that it allows us to introduce real tissue replacements for damaged tissues, rather than artificial replacement implants. Currently stem cell therapy for dogs is being used with some success to treat bone, joint, and ligament problems.

The technology involves the use of adult stem cells, which can be easily obtained, without any harm to the host animal from which they are taken. A small amount of adipose (fat) tissue is surgically removed from a host animal and a centrifuge is used to separate the stem cells from the fat tissue. The stem cells collected are then injected directly into the injured joint, ligament, or bone where they quickly begin to grow, replacing the damaged tissue with new, healthy tissue.

Stem cell therapy holds immense promise for becoming a viable treatment option for a variety of health issues dog’s face.

At Prestige Animal Hospital, we believe that providing your canine companion with the highest quality medical care means being on the cutting edge of the latest technologies and procedures. This is reflected in every aspect of our approach to stem cell therapy for dogs. Our facilities feature state-of-the-art equipment, and our veterinary team stays up to date on the latest canine stem cell research available.

The ideal candidate for canine stem cell therapy is a dog in otherwise good health that suffers from arthritis or hip dysplasia, and who doesn’t respond well to his or her medication. Or, a dog whose quality of life might further suffer due to invasive surgical procedures. Because canine stem cell therapy uses the patient’s own tissues, a canine must be in overall good health in order for any collected stem cells to be effective.

Canine stem cells are collected through removing either fat cells, or various other applicable tissues from a dog’s body. Within these tissues and cells exist regenerative cells that are known colloquially as dog stem cells. The regenerative cells that are collected do several things:

The best part is that canine stem cells are not synthetic cells being added to a living, biological organism. Rather, canine stem cells are a dog’s own natural healing cells, Because of this, there is much less chance of rejection or adverse interaction, and there are also fewer potential side effects.

Due to the infancy of canine stem cell research and therapies, there is not yet a large body of information about possible adverse side effects. Just like any medical procedure, the risk for adverse side effects from dog stem cell therapy are ever-present. Risks could be associated with the tissue removal procedure, or from a patient’s body rejecting the newly placed cells. Other issues with stem cell therapy for dogs include its effectiveness when used alone. This is because the prescription of traditional medication will accompany stem cell therapy procedures.

Deciding whether or not stem cell therapy for dogs is the right choice for your beloved canine companion is a very personal one. No matter what route you choose to take, we are here to help you make the decision that is in the best interest of your dog, and also that fits your budget and lifestyle.

At this time, most stem cell research for dogs currently focuses on treating bone, joint, and ligament problems, rather than treating more advanced illnesses and diseases. We are extremely confident that the day will come when many canine illnesses and diseases can be treated through advances in canine stem cell research.

One thing seems to be clear, stem cell research for dogs promises to revolutionize the veterinary industry, and is already showing good results with canines that have been treated with dog stem cells to repair joints, bones, or ligaments that have been damaged by injury or disease. We are here to help educate you about the latest canine stem cell research advances, and answer any other questions about dog stem cell therapy you might have.

If you would like to discuss how stem cell therapy can benefit your dog, please contact us to schedule an appointment. Our veterinary team can help you decide whether or not canine stem cell therapy is the right option, including discussing cost and prognosis.

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Stem Cell Therapy For Dogs – What you should know

Stem Cell Research On Dogs – Tufts Your Dog Article

Features June 2013 Issue Looking at ways to improve dogs health and lengthen their lives with stem cell studies.

Just as stem cell research is ushering in a new wave of scientific exploration to heal sick people, studies examining the workings of stem cells are underway for treating sick dogs. To find out just where things are at with stem cell research on dogs behalf, we talked with the Director of the Regenerative Medicine Laboratory at Tufts Cummings School of Veterinary Medicine, Andrew Hoffman, DVM, DVSc, DACVIM.

Andrew Hoffman, DVM, DVSc, DAVIM, Director of Tufts Cummings School’s Regenerative Medicine Laboratory, with a friend.

Your Dog: Is stem cell research for dogs subject to the same ethical considerations as for people? For instance, are dog embryos being created just for their stem cells?

Dr. Hoffman: Actually, very little in the field of stem cell research, either for dogs or for people, has anything to do with growing embryos that are then destroyed.

Your Dog: But isnt that how stem cells are grown?

Dr. Hoffman: For all intents and purposes, no. Its true that a developing embryo, no matter what the species, contains stem cells cells that are undifferentiated and therefore arent yet slated to become specific tissue in the body. But its not true that stem cells can be harvested only from developing embryos and nowhere else.

Your Dog: Where, then, can stem cells be obtained for research, or, when their uses are understood and confirmed, for curing or treating dire diseases?

Dr. Hoffman: Common sources include umbilical tissue, bone marrow, and body fat.

Your Dog: Body fat?

Dr. Hoffman: Yes. A developing embryo has three different types of cells, that is, three major embryonic layers of cells that lodge in different tissue once the animal (or person) is developed. The outer layer of cells is the ectoderm, which later forms skin, nerves, and parts of the eye. In the middle come the mesenchymal cells that layer is called the mesoderm and innermost, the endodermal cells.

The most commonly used stem cells in both animal and human research are those which are believed to arise from the middle mesoderm layer, which in mature animals and people can be found in bone marrow, fat stores, and connective tissues of all organs. Theyre the ones that are easiest to grow and harvest in large numbers and then package and prepare for therapeutic application. The cells of the other two layers are more difficult to grow to sufficient numbers, and they also cause a greater immune response in the recipient; theres a greater chance of rejection. So in part, the field as a whole has gone down the easiest path, and in part has selected a cell type that is better tolerated by recipients.

Fortunately, the cells of this middle layer the mesenchymal stem cells, or MSCs, as we call them have demonstrated that they have really broad repair potential. They release a lot of chemicals that stimulate the growth of healthy cells in the body. They stimulate wound repair. MSCs are the golden boys of stem cell therapy right now. There are very few stem cells trials right now that arent using them.

Your Dog: Are you saying that stem cells from fat tissue can be taken from a dog and then injected into a sick part of her body, where they grow into healthy cells to help specific body tissue or a diseased organ repair itself?

Dr. Hoffman: Many people have a notion that stem cells have the capacity to convert themselves into a variety of cell types after transplantation and thus resupply cells in damaged tissues such as kidney cells or heart cells or bone. However, only a very small amount of stem cell research right now is directed at what we call engraftment stem cells attaching to particular tissue or organs and then maturing into cells of those organs. Thats not to say that engraftment isnt of great interest. Even here at the veterinary school at Tufts, we have research in place to regrow a lung, a situation that requires engraftment, cell conversion (differentiation), and cell division (proliferation). Scientists at other research institutions are looking at regenerating heart muscle cells or producing retinal cells that engraft and live on in the eye.

This type of research on stem cells as replacement cells is, however, a small fraction of the research that goes into therapies. Certainly, clinical trials are not focused on using stem cells to replace damaged cells, for example, to get a paralyzed animal [or person] to walk again by injecting stem cells into the spinal cord that turn into nerve cells. This is coming; its only a matter of time before scientists perfect growing up a patients own cells to rejuvenate a part of the body that no longer works, or no longer works properly. Currently, though, the majority of stem cell clinical trials are just about getting stem cells to influence damaged or reserve (healthy) cells in a particular part of the body by releasing signals in the form of chemicals. Thats where the momentum is for the foreseeable future. Stem cells in mature fat tissue or bone marrow couldnt even change into other types of cells; thats where youd need more primitive cells such as embryonic or now, reprogrammed embryonic-like cells.

Your Dog: But why would you want stem cells to just release chemicals? What good would that do?

Dr. Hoffman: Chemicals sent forth from MSCs that middle layer of stem cells lodged in fat tissue and bone marrow have the ability to stimulate repair and regeneration of cells already in place. For example, they stimulate new blood vessel formation, or better closure of wounds. Thats currently where their value lies.

Your Dog: Can you give an example of the specific research taking place?

Dr. Hoffman: We have a faculty member at the Cummings School who has just started a clinical trial in which shes looking at the impact of stem cells on the survival of dogs that have life-threatening kidney disease. Again, shes not injecting dogs with stem cells that she hopes will engraft onto sick kidneys and take over for the sick cells, proliferating to the point of growing whole new kidney tissue. Its about getting the stem cells in there to exert a positive effect on the compromised cells that need help, or coax healthy cells that remain to replace damaged cells. In this particular case, we hope that the MSCs reduce an over-active immune system that is attacking the kidneys.

We have another faculty member who is starting a clinical trial to treat an autoimmune liver disease that afflicts Labrador retrievers and is generally fatal. She hopes to arrest liver inflammation and cirrhosis, or scarring also by reducing the immune response.

Your Dog: How is the research done?

Dr. Hoffman: The stem cells either get injected intravenously (as is the case in the examples of clinical trials at Tufts), or at the site of the problem. When injected intravenously, cells home to different parts of the body (e..g., lung, liver, kidney) and release chemicals locally to those tissues. In the scenario when cells are injected directly into tissues, the cells remain in a holding pattern in that region and release their beneficial chemicals there.

Your Dog: What happens to the stem cells after that?

Dr. Hoffman: They die, mostly. Within about 72 hours, most MSCs are gone. Thats why theyre not being looked at for engraftment and cell proliferation. They simply dont last long enough. That would take the other kinds of stem cells, the ones that come from embryonic tissue or newly discovered reprogrammed cells. But theyre much harder to harvest and grow. And unless the cells are harvested ahead of time from the recipient patient, theyre much more prone to rejection by the body into which theyre injected. MSCs, on the other hand, are popular in research because theyre almost devoid of the main trigger for starting an immune response. The immune system of the tissue into which they are injected doesnt recognize them as foreign to the point that they can be used almost interchangeably between patients without worry that a big immune response will make an unhealthy dog even sicker than she already is. There are some minor reactions, but theyre so minor that even multiple injections of the cells generally do not incite a negative reaction.

Your Dog: Why would you inject stem cells multiple times?

Dr. Hoffman: Because they dont last long. If you want a long-term beneficial effect, several, sometimes many, doses of stem cells are needed. It might be 10 to 20 million cells per injection, which isnt a lot.

Your Dog: How have results been so far?

Dr. Hoffman: Results in clinical trials to date have been promising but not conclusive. There are a lot more veterinarians using stems cells in their practices than there are study results to support their use. No clinical trial has as yet proven, without a shadow of doubt, a clear benefit. This is largely due to the limited size of studies, and the fact that important controls were left out. These studies are very difficult to do for the simple reason that these are our companion animals, and no one wants to be included in a clinical trial and receive either unproven or ineffective treatments or placebos (which have no effects), and no one wants their pet to experience adverse side effects.

With that in mind, one of my pet peeves is the current high expense for stem cells where they have not yet been shown to be valuable. Many people fork over $1,500 to $2,500 to treat their dog or their horse with stem cells, and we dont have the clinical trial data to support recommending those therapies. This seems to be mostly the case for musculoskeletal treatments to ameliorate, say, the symptoms of arthritis or tendon injuries. Companies claim they are seeing benefits in patients that are treated with their MSCs, but the publications are not there for dogs (or humans, for that matter) to support this claim. Theres some research out there, and its provocative, but there is always a question about how much money is worth spending on an unproven therapy, which is often the case with new therapies in veterinary medicine. At this point the medical community, both veterinary and otherwise, is simply trying to understand the merits of stem cell treatments. Were trying to design trials that are objective and ethical and wont hurt the patient while allowing us to garner the information we need. At this point, we have a lot of grade C and D evidence out there, not a lot of As and Bs.

It is also important to note that, unlike the situation in human medicine, stem cells are unregulated thus far in veterinary medicine, so the safety and efficacy and protocols used to generate cells by companies is not under intense scrutiny yet. But this will also come. Tufts is preparing for this inevitability by producing cells in-house, and working on new methods to improve quality and potency of the cells.

Your Dog: Where do you see the field of stem cell research headed in, say, five or 10 years?

Dr. Hoffman: Its headed toward reprogramming or activating stem cells to think. This is a very new area. The process of reprogramming stem cells so that they become particular types of cells (heart cells, nerves, etc.) was only first written up in 2006. And here we are now, talking about it, not only within the scientific community but in the lay community, too. I suspect that within the next five to 10 years for MSCs and the next 10 to 20 years for reprogrammed cells, application of stem cells to treat or even cure disease will have been proven and documented and used as a conventional part of veterinary practice.

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Stem Cell Research On Dogs – Tufts Your Dog Article

Lex (dog) – Wikipedia

Lex (1999 March 25, 2012) was the first active duty, fully fit military working dog to be granted early retirement in order to be adopted. Working for his United States Marine Corps handler Corporal Dustin J. Lee in the Iraq War, he was wounded in an attack that killed Lee, and subsequently awarded an honorary Purple Heart.

Lee and Lex were stationed in the military police department at Marine Corps Logistics Base Albany, with Lex having deployed to Iraq previously with a different handler. Lee had adopted his previous canine, Doenja, after the dog was retired.[1] They were detached to deploy in late November 2006 as an explosive detection and patrol team for 3rd Reconnaissance Battalion, then part of Regimental Combat Team 6.[2][3] On March 21, 2007, while at a Forward Operating Base in Iraq, they were hit by a 73mm SPG-9 rocket attack. Lee was mortally wounded, while Lex sustained shrapnel wounds.[3] Despite his own wounds, Lex refused to leave Lee and had to be dragged away for corpsmen and other medics to attempt treatment.[4] After returning to the United States in April, he fully recovered from his wounds at Marine Corps Base Camp Lejeune after a twelve-week rehabilitation. He returned to MCLB Albany on July 6 in a full working capacity, although some shrapnel remained lodged in his back, which veterinarians did not remove to avoid causing permanent damage to his spine.

We are proud to have had Lex serve alongside Marines here and around the world. His handlers and his sacrifices will not be forgotten. I am glad to be able to support the Lee family, not just in the adoption of Lex, but also to reestablish their connection to their beloved son Dustin.

Col Christian N. Haliday, CO of MCLB Albany

Lees parents, Jerome and Rachel Lee, appealed to the military to adopt Lex. An online petition[5] was created by Lee’s family, and the case gained national media attention. Congressman Walter B. Jones of North Carolina’s 3rd congressional district (which includes Camp Lejeune) assisted the Lee family with the release and adoption of Lex.[6] Although the United States armed forces do not generally release military working dogs prior to retirement age due to certain requirements (due to 10 U.S.C.2583[7]), there existed a provision to existing law that allows for early retirement of dogs under certain situations, created to allow a wounded Air Force dog handler to adopt her partner before retirement in 2005.[8][9] The legal provision was enacted and Lex was released from active service to be adopted by the Lee family.[2]

Headquarters Marine Corps made a formal request to the Air Force working dog program managers at Lackland Air Force Base in November, which was approved on December 6. Lex was released from five years of active service and turned over to the Lee family at a ceremony at MCLB Albany on December 21, 2007, under national media spotlight. He was 8 years old at the time. Afterward, Lex began to visit VA hospitals to comfort wounded veterans and assist in their recovery process. On February 16, 2008, the Military Order of the Purple Heart Chapter #566 presented Lex with a commemorative Purple Heart at a ceremony held at the Air Force Armament Museum at Eglin Air Force Base.[10] On September 24, 2008, the American Kennel Club announced that Lex won the seventh Law Enforcement AKC Award for Canine Excellence.[4] On March 19, 2010, MCLB Albany’s base dog kennel was named in honor of Lee, with Lex in attendance.[3]

After his adoption, Lex struggled with mobility issues due to his injuries and approximately 50 pieces of shrapnel that remain in his body, despite treatment at Mississippi State University’s College of Veterinary Medicine. On November 16, 2010, Lex began treatment at Georgetown Veterinary Hospital with a course of Vet-Stem stem cell regenerative therapy, with assistance from the Humane Society of the United States and Kentucky Congressman Ed Whitfield.[11]

Lex died on March 25, 2012 as a result of cancer.

Banner at Lex’s adoption ceremony

CBS News interviews the Lee family and Marine officials at Lex’s adoption ceremony

Police Officer Reynolds prepares to turn over Lex to the Lee family

Jerome Lee receives Lex at the adoption ceremony

Jerome Lee and Lex at the adoption ceremony

Jerome Lee plays with Lex after the adoption ceremony

The Lee children play with Lex after the adoption ceremony

The Lee family with Lex after the adoption ceremony

Lex awarded an honorary Purple Heart

http://www.sunherald.com/2012/04/20/3895746/slain-marines-service-dog-dies.htmlThis article incorporatespublic domain material from websites or documents of the United States Marine Corps.

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Lex (dog) – Wikipedia

Stem cell research | Animal Health Trust

Stem Cell Research

INTRODUCTION

Stem cells have the ability to self-renew and to turn into other types of cells. We are carrying out research into their potential use in veterinary medicine.

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CURRENT RESEARCH

We are currently researching stem cell therapy for horse tendon injuries and fractures in Thoroughbreds.

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SCIENCE PUBLICATIONS

Read our latest publications on our stem cell research.

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MEET THE TEAM

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CONTACT US

If you want further information on the work we’re doing into stem cell uses, then please do get in touch.

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Stem cells have the ability to self-renew to generate more stem cells and to turn into other types of cells. Broadly, stem cells can be classified into two groups; multipotent stem cells and pluripotent stem cells. Multipotent or adult stem cells exist in many different tissues and can only turn into a limited number of cell types. In contrast pluripotent or embryonic stem cells exist at the very earliest stages of development and can turn into every cell type of the body.

We are carrying out research to use stem cells in veterinary medicine. To date our research has focused on horses and dogs and we have derived pluripotent stem cells from horse embryos and multipotent stem cells from adult horse and dog tissues.

Horse embryo-derived stem cells (ES cells) can be grown indefinitely in the laboratory, express markers associated with pluripotency and can turn many different types of cells. This means they have the potential to be used therapeutically to help regenerate damaged tissues. We have also made horse and dog induced pluripotent stem cells (iPS cells) from adult cells by reprogramming them back to a state where they resemble ES cells. Like ES cells, iPS cells have the potential to be used therapeutically. Furthermore, iPS cells can be isolated from horses and dogs with inherited diseases in order to provide a tool to study that disease in the laboratory.

Horse multipotent mesenchymal stem cells (MS cells) can be isolated from many tissues including the bone marrow and fat tissue of adult horses and the umbilical cord blood of new-born foals.

Tendon injuries occur commonly in racing and sport horses and because the injuries heal through the formation of scar tissue instead of normal tendon tissue they are associated with a high rate of re-injury. Stem cells may help to bring about normal tendon regeneration and therefore reduce the frequency of re-injury. Our research aims to understand the mechanisms by which different types of stem cells work so that we can produce an optimised stem cell therapy for horse tendon injuries.

In current clinical practice horses are treated with their own mesenchymal stem (MS) cells. This requires each horse to have a tissue sample isolated and results in a delay while the cells are processed and grown to sufficient numbers for injection into the tendon. The advantage of embryo-derived stem (ES) cells is that they grow indefinitely in the laboratory and may therefore provide an off the shelf source of cells for treating injuries.

We have shown that ES cells can be injected into the damaged tendons of horses without any undesirable side effects being detected in the 3 month period studied. The ES cells survive in high and stable numbers in the tendon and turn into tendon cells. In the laboratory we have established a 3D culture system to generate artificial tendons from ES cells. We are using this system to determine the signals which drive ES cells to turn into tendon cells to ensure that therapies which use ES cells in the future will be safe and effective.

In contrast to ES cells, MS cells do not turn into tendon cells following their injection into the tendon. However, we have shown that horse MS cells are able to reduce an inflammatory response and they may produce other signals which act to stimulate better tissue regeneration by the bodys own cells.

Fractures caused by bone overloading (as opposed to a direct trauma) are common in racing Thoroughbreds. The risk of fractures is affected by various environmental factors but previous work at the AHT has shown that there is also a genetic risk to fracture in Thoroughbreds.

We are now using iPS cells from horse at high and low genetic risk of fracture to generate bone in the laboratory. This will allow us to determine the biological mechanisms which are affected in high risk horses and understand why these horses are predisposed to fracture.

In the future this will enable the design and application of management techniques to minimise the risk of fracture in horses.

The cornea allows light to be transmitted into the eye. In corneal stromal dystrophy, fat deposits inside the tissue and can lead to ulceration and impaired sight. It occurs in many dog breeds and there are no treatment options available. Corneal transplantation is hampered by a shortage of donor material.

We are carrying out research to study corneal stromal stem cells and produce corneal stromal cells in the laboratory from dog induced pluripotent stem cells (iPSCs). These cells will provide a new tool to enable future studies of this disease to better understand what causes it and if it could be prevented.

The production of corneal cells may also provide a new transplantation therapy for dogs with corneal damage resulting from injury or disease. This work is being performed in collaboration with Professor Julie Daniels, Institute of Ophthalmology, University College London.

Paterson, Y. Z., Kafarnik, C. & Guest, D. J. (2017) Characterisation of companion animal pluripotent stem cells. Cytometry A. doi: 10.1002/cyto.a.23163.

Bavin Emma P., Atkinson Francesca, Barsby Tom, and Guest Debbie J.. Stem Cells and Development. January (2017) Scleraxis Is Essential for Tendon Differentiation by Equine Embryonic Stem Cells and in Equine Fetal Tenocytes

Bavin, E. P, Smith, O., Baird, A.E, Smith, L.C., & Guest, D.J. (2015) Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells. Frontiers in Veterinary Science, 2, 55.

Broeckx, S.Y, Borena, B.,Van Hecke, L., Chiers, K., Maes, S., Guest, D.J., Meyer, E., Duchateau, L., Martens, A., & Spaas, J.H. (2015) Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model. Cytotherapy. 17(10):1434-46

Baird, A.E.G, Barsby, T. & Guest, D.J. (2015) Derivation of canine induced pluripotent stem cells. Reproduction in Domestic Animals. ;50(4):669-76.

Paterson Y. Z., Rash, N., Garvican, E. R., Paillot, R., & Guest, D.J. (2014) Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro. Stem Cell Research and Therapy. 5, 90.

Barsby, T., Bavin, E. & Guest, D. J. (2014) 3-Dimensional Culture and Transforming Growth Factor Beta3 Synergistically Promote Tenogenic Differentiation of Equine Embryo-Derived Stem Cells. Tissue Engineering Part A. 20, 2604-2613.

Broeckx, S., de Vries, C., Suls, M., Guest, D. J., Spaas, J. H. (2013) Guidelines to Optimize Survival and Migration Capacities of Equine Mesenchymal Stem Cells. J Stem Cell Res Ther 3: 147.

Barsby, T. & Guest, D. J. (2013) Transforming Growth Factor Beta3 Promotes Tendon Differentiation of Equine Embryo-Derived Stem Cells. Tissue Engineering Part A. 19(19-20)2156-2165.

Spaas, J. H., Guest, D. J., Van de Walle, G. R. (2012) Tendon Regeneration in Human and Equine Athletes: Ubi Sumus-Quo Vadimus (Where are We and Where are We Going to)? Sports Medicine. 42(10)871-890.

Guest, D. J., Smith, M. R. W. & Allen, W. R. (2010) Equine embryonic stem-like cells and mesenchymal stromal cells have different survival rates and migration patterns following their injection into damaged superficial digital flexor tendons. Equine Veterinary Journal. 42(7), 636-642.

Guest, D. J., Ousey, J. C., and Smith, M. R. W. (2008) Defining the expression of marker genes in equine mesenchymal stromal cells. Stem Cells and Cloning: Advances and Applications. 1, 1-9.

Guest, D. J., Smith, M. R. W., and Allen, W. R. (2008). Monitoring the fate of autologous and allogeneic mesenchymal progenitor cells injected into the superficial digital flexor tendon of horses: preliminary study. Equine Veterinary Journal. 40, 178-181.

Guest, D. J. and Allen, W. R. (2007). Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts. Stem Cells and Development. 16, 789-795.

Greenway, D. J. & Allen, W. R. (2007). Horse stem cells in development and therapies. Reproduction in Domestic Animals. 42(Suppl. 2) 68-68.

For further information please contact

Dr Debbie Guest

debbie.guest@aht.org.uk

+44(0)1638 750000 ex. 1283

Write to us at:

Animal Health Trust

Lanwades Park

Kentford, Newmarket

Suffolk

CB8 7UU

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Stem cell research | Animal Health Trust

Snuppy – Wikipedia

Not to be confused with Snoopy.

Snuppy (Korean: a portmanteau of “SNU” and “puppy”; April 24, 2005May 2015)[1] was an Afghan hound, credited with being the world’s first cloned dog. The puppy was created using a cell from an ear from an adult Afghan hound and involved 123 surrogate mothers, of which only three produced pups (Snuppy being the sole survivor). Department of theriogenology and biotechnology at Seoul National University for cloning Snuppy was led by Woo Suk Hwang. Snuppy has since been used in the first known successful breeding between cloned canines, after his sperm was used to artificially inseminate two cloned females, which resulted in the birth of 10 puppies in 2008.

After Dolly the sheep was cloned in 1996, scientists had managed to clone numerous other animals, including cats, cows, gaur, horses, mice, mules, pigs, rabbits and rats[2] but had been unable to successfully clone a dog due to the problematic task of maturing a canine ovum in an artificial environment.[2] After several failed attempts by other scientists, Woo Suk Hwang, a lead researcher at Seoul National University, was able to successfully create a clone using tissue from the ear of a 3-year-old Afghan hound.[3][4] 123 surrogate mothers were used to carry the embryos, of which 1,095 were implanted, the procedure resulted in only three pregnancies; one resulted in a miscarriage, the other pup was born successfully but died of pneumonia three weeks after birth, the successful clone was carried by a Labrador Retriever.[2] From the original 1,095 embryos to the final two puppies, this placed the success rate of the project at less than two tenths of a percent.[3] Snuppy was named as a portmanteau of the initials of the Seoul National University (SNU) and the word “puppy”.[5]

As the eggs in a female canine are only fertile during the estrus phase of the estrous cycle, the eggs could only be harvested during a three-week period each year. Due to complexities with removing eggs from canine ovaries the eggs had to be extracted from the oviduct, which required constant monitoring to achieve.[6] The nucleus of each egg was replaced with the cell from the ear of the adult dog and then electrified and fused using a chemical reaction. The embryos were then transferred to the surrogate dogs. Three of the surrogate mothers became pregnant and two successfully gave birth. Snuppy, the first to be born, survived while the other died two weeks after birth.[6] This process of cloning Snuppy took nearly three years of intensive effort.[7]

Snuppy was named as Time Magazine’s “Most Amazing Invention” of the year in 2005.[3] Particular recognition was given to the cloning technique used in the process, which Time stated was “embodied by a history-making puppy”. Despite numerous labs performing mammalian cloning, they cited that Hwang’s team and Snuppy were “extraordinary”.[6][8] The experiment was criticised by Robert Klitzman, director of Columbia University’s Masters in Bioethics program, who cited that the process raised the question of if humans are “just a mass of cells and biological processes?”[6] Hwang himself criticised the process, stating that it did not bring science any closer to human cloning and the complexities, coupled with the low success rate (one in 123), did not make it ethical to clone family pets.[2] Ian Wilmut, the scientist behind the successful cloning of Dolly the sheep, said that the successful cloning of Snuppy proved that any mammal could be cloned in the correct environments and that a global ban on human cloning needed to be quickly implemented because of this.[2] The Kennel Club criticised the entire concept of dog cloning, on the grounds that their mission is to “To promote in every way the general improvement of dogs” and no improvement can occur if replicas are being created.[4]

Between late 20052006 Hwang was accused of a series of misconducts. The first allegations related to his work prior to Snuppy; the claim that he had successfully cloned a human embryo. The charges alleged Hwang had paid for egg donations and that some of eggs came from his employees, which constitute serious breaches of the code of bioethics. It was later found that photographs he published did not depict what was suggested and that most of the stem-cell lines he claimed to have created were not clones at all.[9] This brought serious doubts onto the validity of Snuppy, which Hwang consistently claimed was a genuine clone. Hwang hired HumanPass Inc., a Korean DNA lab to investigate Snuppy, who found that Snuppy was authentic. The findings by HumanPass were dismissed on the grounds that they were employed by Hwang, and a panel at the Seoul National University ordered their own investigation.[10] The investigation found that, despite his fabrications in previous projects, Hwang’s research related to Snuppy was accurate and Snuppy was a clone of the adult Afghan hound.[11] As a result of his forgeries, Hwang was indicted for fraud and dismissed from the university.[12]

Veterinary professor Byeong Chun Lee took over leadership of the team behind Snuppy.[13] In 2008, Snuppy became involved in the first known successful breeding between cloned canines, after sperm taken from Snuppy was used to artificially inseminate two cloned females, which resulted in the birth of 10 puppies.[14] Nine of the puppies survived.[15] The SNU team, under Lee, have gone on to successfully clone over 30 dogs and five wolves.[16] After successfully breeding the cloned wolves, Lee claimed that the ability to breed cloned canines makes it possible for working dogs which are usually sterilised before training, such as sniffer dogs and guide dogs, to reproduce.[17] SNU, which claimed to own the patent for the process used to clone Snuppy, formed a license agreement with RNL Bio, a commercial pet cloning company. Hwang entered into a partnership with RNL Bio’s competitor, BioArts International, which caused an ongoing legal battle into who owns the patent rights[12][18] although Bio Arts withdrew from dog cloning in 2009.[19] RNL completed the first commercial cloning in August 2008[20] but ran into financial trouble in 2013.[21]

The world’s first cloned sniffer dogs (all of which are named Toppy) were put to work by South Korean customs in July 2009.[22]

Supporters of Hwang founded a company called Sooam Biotech where Hwang developed proprietary techniques[23] based on a licence from ViaGen’s subsidiary Start Licensing (which owns the original Dolly patent[24]) and created cloned dogs for owners whose dogs had died, charging $100,000 a time[25] Sooam Biotech was reported to have cloned 700 dogs by 2015[25] and to be producing 500 cloned embryos of various species a day in 2016.[26]

Snuppy died in May 2015 at the age of 10.[27]

Link:
Snuppy – Wikipedia

Stem Cell Docu Series

Jeff Hays is a filmmaker and a serial entrepreneur. He began making films back in the late nineties. Hes best known for Fahrenhype 9/11 (2004), a response to Michael Moores Fahrenheit 9/11, and On Native Soil (2006), a documentary focusing on the passionate demand of the surviving family members of 9/11 victims for an official investigation into the 9/11 attacks. This documentary, which was narrated by Kevin Costner and Hillary Swank was short-listed for an Academy Award in 2005.

Hays recent health documentary releases are Bought, Doctored, Undoctored, Rigged 2016 and the GMOs Revealed and Vaccines Revealed series programs. Hays explains, In recent years my eyes have been opened to alternative healthcare options. The immediate increase in health I experienced personally after starting down this road is something Ive just got to share with people.

Hays most recent release is Christ Revealed, a 9-episode docuseries featuring exclusive expert interviews and a stunning tour of Israel.

Also, Chasing the Scream, the New York Times Best Selling book by Johann Hari is being brought to life by Jeff Hays Films and is now in pre-production with Academy Award winning co-producers and Academy Award nominated directors attached.

Two additional documentary series from Jeff Hays Films are being released in early 2018. The Healing Miracle: the truth about stem cells, and The Real Skinny on Fat, the truth about weight loss.

A pet project is Movie Maker Academy where Hays is on the faculty teaching filmmakers and entrepreneurs how to create successful donor crowdfunding projects based on his 7-figure success in crowdfunding.

Hays lives in the mountains of Utah with his dog Abby and works from his office in NY. Hes the father of 9, and grandfather of 12, with new ones annually

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Stem Cell Docu Series

Stem Cell Therapy for Dogs and Cats – Vet4Bulldog

Stem Cell Therapy for dogs and cats, bulldogs and other pets.Dr. R. Kraemer, veterinarian and owner of Vet4HealthyPet Advanced Medical Care Pet hospital located at 434 South Tustin Street at theCity of Orange inOrange County, California,has provided veterinary services since 1992 for dog, cat and other pet owners and is now a leading provider for stem cell therapy for dogs and cat. Due to increased demand, Dr. Kraemer is expanding the offer for this groundbreaking, regenerative medicine and cryobanking to pet owners from anywhere in the State of California, as well as those out of state who are unable to find Stem Cell treatment/therapy for dogs and cats (their pets) from their local veterinarians.

PDF DOWNLOAD Click Here

Stem Cells for dogs and cats are extracted from your pets fatty tissue, and processed with Platelet Rich Plasma (PRP)into an injectable solution, which is then activated using LED Light technology. Following activation, the solution is then injected intra-articular (i.e. directly into arthritic joints) as well as intravenously. This is a same day procedure with no serious side effects, no extensive recovery time, or confinement needed.

Stem Cell Therapy for Dogs and Cats: Dr. Kraemer is a leading provider for Stem Cell Therapy treatment for dogs and cats (pets)

Economical Quality of Life Enhancement Minimal Confinement Minimal Post-Op Recovery Time No Post-Op Rehabilitation

Stem Cell Therapy by Dr. Kraemer for Arthritis, Hip Dysplasia and Degenerative Joint disease in Pets

Promising results forstem cell therapy for dogs and catscurrently shown in Clinical trials-Case studies-Peer review-Compassionate use:

Dr. Kraemer extracting the stem cells from his pet patient adipose tissue

I sat down for a Q&A with three bulldog rescue members who whose pets I have treated with stem cells for various medical conditions. Southern California Bulldog Rescue is a non for profit rescue organization dedicated to providing deserted and abused bulldogs with medical care, housing, and placement with new families. I have a close relationship with SCBR and have been providing medical care for their rescued pets for many years andshelter and at my new 434 south Tustin street, orange, Ca. veterinary hospital. If you would like to help SCBR and bulldog rescue, we invite you to join our U4B Pledge. SCBR also accepts donations, which are deductible to the full extent of the law.

Vicky (SCBR): Dr. Kraemer what are stem cells?

Dr. Kraemer Answers: Stem cells are the bodys repair cells we all have waiting to be called on when injury occurs and as our body ages (wear & tear). Stem Cells have the ability to divide and differentiate into many different types of cells based on where they are needed throughout the body. Stem cells can divide and turn into tissues such as skin, fat, muscle, bone, cartilage, and nerve, to name a few. They even possess the ability to replicate into organs such as the heart, liver, intestines, pancreas, etc.

Ronnie (SCBR): What the term RegenerativeMedicine means?

Dr. Kraemer Answers:Its theuse of our own cells to augment or stimulate the bodysnatural healing processes. Regenerative medicine is the process of creating living, functional tissues torepair or replace tissue or organ function lost due to age, disease, damage, or congenital defects.

Nicole (SCBR): A friend of mine had banked her newborn child stem cells from her own placenta and umbilical cord, are yours the same kind of stem cells?

Dr. Kraemer Answers: There are two basic types of stem cells; embryonic and somatic (adult). Embryonic Stem Cells are found in the placenta, umbilical cord and embryo, these cells are called totipotent, which means they have the ability to reproduce into any mature cell type. While Embryonic Stem Cells offer the greatest potential in healing, there are obviously moral and ethical concerns in harvesting these cells.

The second type of stem cell is the Adult Stem Cell, these stem cells are called multipotent, which means they can differentiate into closely related cell lines, but they are not capable of creating a complete organ. Adult Stem Cells are found in the bone marrow, adipose tissue (fat), skin, liver, blood vessels, and neurons. Contrary to embryonic stem cells, there are NO moral or ethical concerns in harvesting Adult Stem Cells, activating them, and reintroducing them back to the patient in areas where healing and regeneration is needed.

Ronnie (SCBR): So Dr. Kraemer why you prefer extracting the stem cells from adipose (fat) tissue?

Dr. Kraemer treating a dog patient with stem cell therapy for joint disease and arthritis

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): Adult stem cells are highly concentrated in the fat tissue. There are 50 to 1,000 times more stem cells in the fat than the bone marrow. At this concentration, it is no longer necessary to culture the stem cells to acquire the necessary cell numbers to make a healing impact. Compare to harvesting from bone marrow Adipose tissue is easier to get, less painful and involve lower risk while yielding many more stem cells compared to bone marrow. On matter of speed and degree of risk this procedure (i.e. to extract fat from your pet) is much quicker and less invasive than even a routine spay. The stem cells are contained within a pool of cells in the fat termed the Stromal Vascular Fraction (SVF)which includes bone marrow stromal cells and mesenchymal cells along with many beneficial proteins that on cellular level encourage:

Stem Cell in action (microscopic view)

Nicole (SCBR): Dr. Kraemer, can you explain how Stem Cells work and how would they help our pets?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): Stem Cells work like heat-seeking guided missiles, only in this case they are seeking damaged, injured, aging cells in urgent need of repair. Their repair abilities are multi-factorial which include among other,

Adult stem cells are capable of dividing into many different cell types. With this capability, we can use them as a treatment for joint injuries, ligament, tendon damage, and fractured bones. Stem Cell therapy also has shown promise for renal disease, dermatological conditions, and muscle tear repair.

Ronnie (SCBR): Which dogs and cats are the best candidates for Stem Cell Treatments?

Dr. Kraemer Answers:Dogs and cats that have not responded well to non-steroidal anti-inflammatory (NASID) drugs and other pain medication and continue to suffer from debilitating pain.

Stem Cells in action at the cellular level

Vicky (SCBR): Dr. Kraemer what makes the stem cell technology you are implementing superior to other stem cell therapies currently available in veterinary practice?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): I am one of the few doctors in our area who are certified by MediVet Americas technology. MediVet is a patented L.E.D. technology incorporating Platelet-Rich Plasma (PRP) the same treatment used by many sports professionals. MediVet America is able to acquire the most living stem cells of any company currently offering this technology. There is no stem cell lost due to transportation and poor handling since I have my own in-house fully equipped specialized laboratory. That is a big advantage over all the other systems since they require you to ship the fat to an outside lab for the extraction and processing. This delay will cause cell death and force the client to bring his pet back the next day for the final injectable process (i.e. treatment) .

Nicole (SCBR):Can stem cells be banked and used again to treat future problems and repeat treatments?

Dr. Kraemer answers (Stem Cell Therapy for dogs and cats):In collaboration with MediVEt America Ioffer Cryobanking, where you can store extra cells from the procedure for future use. Many of our clients whove had their pet treated with stem cells opted to cryobank some or their pets stem cells for future treatments. Most of the time we can get up to six additional treatments from the cryobanked stem cells.

Ronnie (SCBR): Dr. Kraemer, can you tell us how long the procedure takes and what it entails?

Dr. Kraemer holding a Platelet Rich Plasma (PRP) vial ready to be mixed up with his patient stem cells

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): The day of the procedure you will bring your pet to our Animal Hospital. We will first fit them with an IV catheter through which we will administer fluids and pre-op pain medication. We will then anesthetize your pet just long enough (about 15 min) so as to alflow me to surgically remove a couple of tablespoons of fat. This is a quick and simple procedure that is generally easier than performing a typical spay. Dr. Kraemer and his staff at the Animal Hospital will then process the fat at the in-house lab in order to extract the stem cells using MediVet patented kit. They will then be mixed withPlateletRich Plasma extracted from your pets own blood. The whole process takes a couple of hours. Just before those final highly concentrated, pure stem cells are ready to be injected, we will activate them to become supercharged (i.e. super-excited) with a special L.E.D light. During the final step your pet will be mildly sedated, and will receive pain medication for comfort. The stem cells will then be administered into the affected joints and/or into the bloodstream intravenously. It is important that you do not feed your pet the night before the procedure

Vicky (SCBR): Dr. Kraemer, do you guarantee success, and how soon is it before most pet owners will notice an improvement?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): There are no guarantees, as each pet is different. Nationwide, 95% of procedures for osteoarthritis and hip dysplasia have shown clinical improvement. Some pet owners report seeing a difference in as little as a week. Others do not see a change for a month or two. If your pet is going to show improvement, we expect that it will occur within the first 90 days following treatment. Really bad arthritis may require multiple injections, so banking your extra cells is always a good idea.

Nicole (SCBR): Dr. Kraemer can you go over any safety concerns some pet owners might have?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): As with any procedure that involves anesthesia, even one with as short of duration as this one, there is always some risk factor involved. However, the stem cells are coming from your pet (Autologous) and are being re-administered back to your pet. There is no risk of an allergic reaction, no cell rejection or disease transmission. Rarely, there might be a mild immune reaction in the injected joint that should subside within a day or two.

Ronnie (SCBR): I think most of us who have had our pet treated are excited about this new age therapeutic modality, yet cost is still an important factor in a pet owners final decision. Can you tell us how much will this cost?

Dr. Kraemer actives (super charges) his patient stem cells with LED light

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): Think about it this way, a young pet diagnosed with osteoarthritis may require surgical intervention, rehabilitative therapy, and potential lifelong prescription for pain medication. The cost of Stem Cell therapy would be less, and cells can be stored for the lifetime of the animal and used again at a much lower cost than the initial process. Its also important to remember the potential complications with surgeries like infection, implant failure and soft tissue trauma, plusrecovery is lengthy aftersurgery and requires weeks of strict confinement. And in regards to long term painmanagementmedication, even the new generation pain relief drugs can have adverse effects, requiremonitoring, and, in some cases, can lead to serious health complications. Our Pet Hospital offers two different sized stem cell kits for pets.

The difference in cost between the two kits is ONLY 300 dollars (i.e. double the stem cells for only an additional 15% extra above the small kit cost). Hence, for most cases the LARGE stem cell kit is by far the better choice, allowing us to inject the maximum amount to multiple joints and still have plenty left to give intravenously or/and store (cryobank) some for years to come.

Ronnie (SCBR): Speaking about cost, is stem cell therapy covered by the various pet insurances?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): Great question and very timely. Yes most pet insurance companies cover stem cells in treatment as long as the disease itself is covered by the policy.

Nicole (SCBR): Lately I have been hearing on the news about many new life saving medical applications and discoveries with stem cells. How do you see the future of stem cell therapy?

Dr. Kraemer Answers (Stem Cell Therapy for dogs and cats): I am glad you brought it up Nicole. Regenerative medicine is in its infancy, and has unlimited potential. In the coming years, I foresee a major advance into every day practice with a much wider range of therapeutic applications available. So I believe it is here to stay. As the body of science in veterinary stem cell therapy increases, I envision more and more pet owners opting for collecting stem cells prophylactically when their pet is being spayed or neutered, and storing the cells for later indications and uses. Future science will likely prove that stem and regenerative cell therapies should be part of multimodal approaches to increase chances for the best outcome for many veterinary patients.

Dr. Kraemer is a leading provider of stem cell therapy for the use of Osteoarthritis injuries and pain in pets

Vicky (SCBR):Thank you, Dr. Kraemer, for your time and for all the help you and your staff have provided our rescue over the years. I also wanted to personally thank you and MediVet of America for what you did for our Piper, if it was not for stem cell therapy she would have not been with us. You made me a believer. Any final thoughts?

Dr. Kraemer (Stem Cell Therapy for dogs and cats): Thank you Vicky for the kind words,it is a true privilege to be part of the SOUTHERN CALIFORNIA BULLDOG RESCUE organization.In veterinary medicine, while many of the compassionate cases outcomes are anecdotal, we still lack solid scientific trials to prove efficiency, though more of them are being conducted now by universities around the country. Stem cells have already shaped the contemporary medicine of humans and should, sometime soon, do the same in veterinary medicine, with more clinical trials done in laboratory settings. I have been treating compassionate cases who came to me after traditional medicine failed, leaving them with euthanasia as the only human option. Such was the case of Vickys (Southern California Bulldog Rescue)Piperan English bulldog who was treated by multiple internal medicine specialists for IBD (inflammatory bowel disease) for over seven months, prescribed multiple immune suppressant steroids, antibiotics, and a handful of other drugs, homeopathic remedies and special diets and yet continued to show no improvements. Piper was down to 23lb, with protruding bones and explosive, non-responsive diarrhea. To read more about Pipers amazing story ,click here.Among many other compassionate cases,I have also treated a 13 year old Degenerative Myelopathy, a grave, non-treatable condition that was sent home after an MRI with no hope. I also treated an English bulldog with non-responsive, erupting and painful interdigtial cysts who developed serious complications due the immune suppressant drugs he was on, and other cases, many of which I will present on this site in coming weeks.

I would like to say that we can all eat healthy, exercise regularly and learn to relax in order to improve our life quality and increase longevity. The same goes for our pets. But as most of us are painfully aware, injuries and illness could happen despite our best efforts to live a healthy lifestyle. Many times surgery will be recommended, and there are certainly many times when surgery is appropriate and needs to be performed. We should all be VERY grateful we have access to this tool. Nevertheless, I am a big advocate and strongly believe in awareness of other, less invasive, safer, often less costly, effective, alternative options. The latest therapeutic modalities like stem cell therapy, Platelet Rich Plasma, and class-4 Cold Laser Therapyare here to stay, and its only the beginning. I predict that in the coming decade, those new age modalities will be main stream and an integral part of every hospitals medical services. With the rise of regenerative medicine it is sufficient to say that you are witnessing the emergence of a completely new age in healing and certainly a total paradigm shift in veterinary health care.

Recognizing the success of regenerative medicine in humans (notable examples include bone marrow transplantation, work done on heart cells, skin and corneal regeneration) there is no doubt in my mind that we will continue to see a robust usage with increasingly wider medical applications. Regenerative medicine is growing rapidly, and it may replace or influence many current treatments giving new and exciting therapeutic modalities to pet owners.

Excerpt from:
Stem Cell Therapy for Dogs and Cats – Vet4Bulldog